{"id":7870,"date":"2026-02-04T11:16:29","date_gmt":"2026-02-04T10:16:29","guid":{"rendered":"https:\/\/goba.eu\/vpliv-olivnih-pescic-na-rast-gob-sitake-lentinus-edodes-in-ucinek-njihovih-polisaharidov-na-razmnozevanje-celic-ccrf-cem\/"},"modified":"2026-05-20T11:13:09","modified_gmt":"2026-05-20T10:13:09","slug":"vpliv-olivnih-pescic-na-rast-gob-sitake-lentinus-edodes-in-ucinek-njihovih-polisaharidov-na-razmnozevanje-celic-ccrf-cem","status":"publish","type":"post","link":"https:\/\/goba.eu\/en\/vpliv-olivnih-pescic-na-rast-gob-sitake-lentinus-edodes-in-ucinek-njihovih-polisaharidov-na-razmnozevanje-celic-ccrf-cem\/","title":{"rendered":"Influence of Olive Oil Press Cakes on Shiitake (Lentinus edodes) Fruiting Bodies Production and Effect of their Crude Polysaccharides on CCRF-CEM Cell Proliferation"},"content":{"rendered":"<figure>\n<figure><\/figure>\n<\/figure>\n<p>Shiitake culinary-medicinal mushroom, <i>Lentinus edodes<\/i>, is a popular species especially used in Asia. It is known for its nutritional value as well as medicinal potential, which is primarily linked to polysaccharides (beta glucans). One of these polysaccharides is lentinan, which is reported to possess antitumor, antibacterial, antiviral, anticoagulatory, as well as wound-healing activities.<span class=\"s2\">\u00a0<\/span>It is also registered as a medicine in some parts of the world. Beta glucans possess the ability to link themselves to immunoglobulins in the blood serum and to stimulate the immune system.<span class=\"s2\">4 <\/span><i>In vitro <\/i>and clinical investigations have revealed that the consumption of fruiting bodies of higher Basidiomycetes can prevent oncogenesis, increase the level of activated macrophages, and lead to shrinkage and disappearance of tumors.<\/p>\n<p class=\"p2\"><i>Lentinus edodes <\/i>(Berk.) Singer fruiting bodies were cultivated on substrates composed of beech sawdust, wheat bran, and calcium sulfate hemihydrate (gypsum), containing different proportions of olive oil press cakes (OOPC).<\/p>\n<p class=\"p2\">We determined the influence of OOPC on fruiting bodies production and proliferation of CCRF-CEM leukemia cells.<\/p>\n<p class=\"p2\">A negative influence of OOPC on mycelia growth and maturation was noticed. When growth medium contained 80% OOPC, fruiting bodies ceased forming.<\/p>\n<p class=\"p2\"><span class=\"s1\"> To investigate the cytotoxicity on CCRF-CEM cells <\/span><i>in vitro<\/i>, cells were treated with crude polysaccharides extracted from <i>L. edodes <\/i>fruiting bodies. Also in this case a negative correlation between OOPC content and cytotoxicity was found.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In this study, we observed a negative effect of OOPC (Olive Oil Press Cakes) added to the substrate on mycelial growth and fruiting body maturation of Lentinula edodes, as well as a negative correlation between the OOPC and the cytotoxic effect on CCRF-CEM cells (leukemia cells).<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1072],"tags":[1058,1119,1056,1128,1120,1121],"class_list":["post-7870","post","type-post","status-publish","format-standard","hentry","category-studies","tag-ccrf-cem","tag-cytotoxicity","tag-lentinula-edodes","tag-medicinal-mushrooms","tag-medicinal-mushrooms-polysaccharides","tag-shiitake"],"acf":[],"_links":{"self":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/7870","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/comments?post=7870"}],"version-history":[{"count":5,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/7870\/revisions"}],"predecessor-version":[{"id":11973,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/7870\/revisions\/11973"}],"wp:attachment":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media?parent=7870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/categories?post=7870"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/tags?post=7870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}