{"id":11875,"date":"2026-05-19T07:49:43","date_gmt":"2026-05-19T06:49:43","guid":{"rendered":"https:\/\/goba.eu\/ovrednotenje-recikliranega-papirnega-mulja-kot-substrata-za-proizvodnjo-lignoceluloliticnih-encimov-pri-glivi-pleurotus-ostreatus\/"},"modified":"2026-05-19T12:10:14","modified_gmt":"2026-05-19T11:10:14","slug":"valorisation-of-deinking-sludge-as-a-substrate-for-lignocellulolytic-enzymes-production-by-pleurotus-ostreatus","status":"publish","type":"post","link":"https:\/\/goba.eu\/en\/valorisation-of-deinking-sludge-as-a-substrate-for-lignocellulolytic-enzymes-production-by-pleurotus-ostreatus\/","title":{"rendered":"Valorisation of deinking sludge as a substrate for lignocellulolytic enzymes production by Pleurotus ostreatus"},"content":{"rendered":"
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Disposal of waste sludges<\/a>\u00a0produced in large amounts in the\u00a0pulp and paper industry\u00a0imposes significant environmental and economical problems. One strategy to address these issues involves revalorization of\u00a0paper mill<\/a>\u00a0sludges<\/a>\u00a0by their application as substrates for microbial production of biotechnologically relevant\u00a0enzymes<\/a>. The application of lignocellulolytic\u00a0enzymes<\/a>\u00a0in paper, textile and\u00a0bioenergy<\/a>\u00a0industries is encouraged in order to decrease chemicals and energy consumptions.<\/p>\n<\/div>\n

In the following work,\u00a0deinking<\/a>\u00a0sludge was assessed as a substrate for production of lignocellulases. Based on the results of growth and activity screenings,\u00a0Pleurotus ostreatus<\/a>\u00a0PLAB was chosen as the most promising candidate among 30 tested strains and its\u00a0secretome<\/a>\u00a0was further studied by quantitative\u00a0enzyme assays<\/a>\u00a0and\u00a0mass spectrometry<\/a>.<\/p>\n

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While\u00a0endoglucanase<\/a>\u00a0and\u00a0xylanase<\/a>\u00a0activities detected in\u00a0P.\u00a0ostreatus<\/em>\u00a0secretome<\/a>\u00a0produced on\u00a0deinking<\/a>\u00a0sludge were similar to activities of cultures grown on other lignocellulosic substrates, average\u00a0laccase<\/a>\u00a0activity was significantly higher (46\u202f000 U\/kg\u00a0DIS<\/sub>). Mass spectrometry identification of the most prominent proteins in the\u00a0secretome<\/a>\u00a0of the target strain confirmed that significant amounts of different lignin-modifying oxidases were produced on this substrate despite its low lignin content, indicating the presence of other inducible compounds.<\/p>\n

The findings of this study suggest deinking sludge may represent a good substrate for fungal production of the aforementioned enzymes with broad biotechnological applications, including bioremediation, paper and\u00a0bioenergy<\/a>\u00a0industries.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Disposal of waste sludges\u00a0produced in large amounts in the\u00a0pulp and paper industry\u00a0imposes significant environmental and economical problems. One strategy to address these issues involves revalorization of\u00a0paper mill\u00a0sludges\u00a0by their application as substrates for microbial production of biotechnologically relevant\u00a0enzymes. The application of lignocellulolytic\u00a0enzymes\u00a0in paper, textile and\u00a0bioenergy\u00a0industries is encouraged in order to decrease chemicals and energy consumptions. In […]<\/p>\n","protected":false},"author":3,"featured_media":11881,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1072],"tags":[1546,1077,1547,1545,1336],"class_list":["post-11875","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-studies","tag-deinking-sludge","tag-fungi","tag-lignocellulolytic-enzymes","tag-paper-mill-waste","tag-solid-state-fermentation"],"acf":[],"_links":{"self":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11875","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/comments?post=11875"}],"version-history":[{"count":5,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11875\/revisions"}],"predecessor-version":[{"id":11929,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11875\/revisions\/11929"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media\/11881"}],"wp:attachment":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media?parent=11875"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/categories?post=11875"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/tags?post=11875"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}