{"id":11843,"date":"2026-05-18T12:43:37","date_gmt":"2026-05-18T11:43:37","guid":{"rendered":"https:\/\/goba.eu\/degeneracija-kulture-zmanjsuje-izrazanje-genov-povezanih-s-spolnim-razvojem-spreminja-tvorbo-metabolitov-in-zmanjsuje-entomopatogeni-odziv-pri-glivi-cordyceps-militaris\/"},"modified":"2026-05-20T11:06:47","modified_gmt":"2026-05-20T10:06:47","slug":"culture-degeneration-reduces-sex-related-gene-expression-alters-metabolite-production-and-reduces-insect-pathogenic-response-in-cordyceps-militaris","status":"publish","type":"post","link":"https:\/\/goba.eu\/en\/culture-degeneration-reduces-sex-related-gene-expression-alters-metabolite-production-and-reduces-insect-pathogenic-response-in-cordyceps-militaris\/","title":{"rendered":"Culture Degeneration Reduces Sex-Related Gene Expression, Alters Metabolite Production and Reduces Insect Pathogenic Response in Cordyceps militaris"},"content":{"rendered":"
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The study \u201cCulture Degeneration Reduces Sex-Related Gene Expression, Alters Metabolite Production and Reduces Insect Pathogenic Response in Cordyceps militaris\u201d<\/em> investigates how long-term laboratory subculturing of Cordyceps militaris<\/em> affects its biological activity, metabolism, and ability to produce important bioactive compounds.<\/p>\n

The researchers found that repeated subcultivation on artificial media gradually led to culture degeneration. Degenerated cultures exhibited significantly reduced production of cordycepin and pentostatin, two important secondary metabolites associated with the biological activity of Cordyceps militaris<\/em>.<\/p>\n

In addition to the decreased production of these compounds, degenerated cultures showed substantial alterations in their overall metabolic profile. Metabolomic analyses revealed reduced levels of numerous metabolites involved in energy metabolism, purine metabolism, and other essential biological pathways. The researchers also observed reduced expression of genes associated with fungal sexual development and fruiting body formation.<\/p>\n

The study highlights that prolonged and repeated laboratory propagation can significantly affect the quality and biological activity of fungal cultures. The findings suggest that proper management of master cultures and careful control of cultivation practices are essential for maintaining culture stability and preserving the production of bioactive compounds.<\/p>\n

The authors further emphasize that the reduction in cordycepin production and the altered metabolic profile were directly associated with culture degeneration, which may ultimately influence the quality of final mushroom-derived products.<\/p>\n

Overall, the study further demonstrates the importance of carefully controlled cultivation procedures, proper preservation of fungal strains, and limiting excessive serial subculturing in the production of high-quality Cordyceps militaris<\/em> products.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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The study \u201cCulture Degeneration Reduces Sex-Related Gene Expression, Alters Metabolite Production and Reduces Insect Pathogenic Response in Cordyceps militaris\u201d investigates how long-term laboratory subculturing of Cordyceps militaris affects its biological activity, metabolism, and ability to produce important bioactive compounds. The researchers found that repeated subcultivation on artificial media gradually led to culture degeneration. Degenerated cultures […]<\/p>\n","protected":false},"author":3,"featured_media":11845,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1072],"tags":[1528,1529,1530,1525,1531],"class_list":["post-11843","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-studies","tag-degeneration","tag-entomopathogen","tag-fungal-sexual-biology","tag-galleria-mellonella","tag-metabolomics"],"acf":[],"_links":{"self":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11843","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/comments?post=11843"}],"version-history":[{"count":4,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11843\/revisions"}],"predecessor-version":[{"id":11931,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11843\/revisions\/11931"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media\/11845"}],"wp:attachment":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media?parent=11843"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/categories?post=11843"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/tags?post=11843"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}