![\"\"](\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/S-kom-sodelujemo.jpg\")
<\/a><\/p>\nOur research focus on the isolation of a diverse range of bioactive compounds from employing conventional crhomatographic techniques, followed by their structural characterization predominantly through nuclear magnetic resonance (NMR) spectroscopy. Hericene A was isolated from the ethyl acetate extract of the H. erinaceus fruiting bodies by performing gravitational chromatography, followed by additional purification on semi-dry flash chromatography.<\/p>\n
Furthermore, erinacines A and C were isolated from the ethyl acetate extract of the mycelium. The extract was initially processed through semi-dry flash chromatography, followed by subsequent purification utilizing preparative high-performance liquid chromatography (HPLC).<\/p>\n
The structures of isolated compounds were determined using 1D (\u00b9H NMR and 13<\/span>C) and 2D (COSY, JRES<\/span>, HMBC, HSQC and NOESY) NMR experiments. These purification methodologies demonstrated high efficiency and cost-effectiveness, resulting in the isolation of pure natural compounds appropriate for subsequent pharmacological investigations.<\/p>\n Future investigations will prioritize the optimization of isolation protocols for various metabolites derived from both the mycelium and fruiting bodies of H. erinaceus, alongside comprehensive characterization and systematic evaluation of their biological activities, with the aim of elucidating potential therapeutic applications in the treatment of neurodegenerative disorders, particularly Alzheimer’s disease.<\/p>\n","protected":false},"excerpt":{"rendered":" Research was funded by Our research focus on the isolation of a diverse range of bioactive compounds from employing conventional crhomatographic techniques, followed by their structural characterization predominantly through nuclear magnetic resonance (NMR) spectroscopy. Hericene A was isolated from the ethyl acetate extract of the H. erinaceus fruiting bodies by performing gravitational chromatography, followed by […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1072],"tags":[1495,1175,1491,865,1493,1492,866,1494,1496,1497],"class_list":["post-11713","post","type-post","status-publish","format-standard","hentry","category-studies","tag-alzheimers-disease","tag-bioactive-compounds","tag-bioactive-metabolites","tag-erinacin-a","tag-erinacines","tag-hericenones","tag-hericium-erinaceus","tag-ischemic-stroke","tag-neurodegenerative-disorder","tag-neurons"],"acf":[],"_links":{"self":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11713","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/comments?post=11713"}],"version-history":[{"count":7,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11713\/revisions"}],"predecessor-version":[{"id":12076,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11713\/revisions\/12076"}],"wp:attachment":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media?parent=11713"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/categories?post=11713"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/tags?post=11713"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}