{"id":11686,"date":"2026-05-13T11:03:14","date_gmt":"2026-05-13T10:03:14","guid":{"rendered":"https:\/\/goba.eu\/dvodimenzionalna-kromatografska-izolacija-visoko-preciscenega-erinacina-a-iz-glive-hericium-erinaceus\/"},"modified":"2026-05-19T12:15:38","modified_gmt":"2026-05-19T11:15:38","slug":"dvodimenzionalna-kromatografska-izolacija-visoko-preciscenega-erinacina-a-iz-glive-hericium-erinaceus","status":"publish","type":"post","link":"https:\/\/goba.eu\/en\/dvodimenzionalna-kromatografska-izolacija-visoko-preciscenega-erinacina-a-iz-glive-hericium-erinaceus\/","title":{"rendered":"Two-Dimensional Chromatographic Isolation of High Purity Erinacine A from Hericium erinaceus"},"content":{"rendered":"<p data-start=\"0\" data-end=\"170\">A simple and robust two-dimensional chromatographic fractionation protocol for the isolation of the neuroprotective compound erinacine A from <em>Hericium erinaceus<\/em> is proposed.<\/p>\n<p data-start=\"0\" data-end=\"170\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-11684\" src=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-13-at-12.14.25-288x300.jpg\" alt=\"\" width=\"445\" height=\"464\" srcset=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-13-at-12.14.25-288x300.jpg 288w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-13-at-12.14.25-287x299.jpg 287w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-13-at-12.14.25-600x626.jpg 600w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-13-at-12.14.25.jpg 621w\" sizes=\"auto, (max-width: 445px) 100vw, 445px\" \/><\/p>\n<p data-start=\"172\" data-end=\"431\">This production platform yielded 19.4 mg of erinacine A from approximately 130 g of mushroom material, with a chromatographic purity of 97.4%. The procedure includes extraction, concentration, fractionation, purification, and characterisation of the bioactive compound.<\/p>\n<p data-start=\"433\" data-end=\"827\">The crude <em>H. erinaceus<\/em> extract was fractionated in the first dimension by normal-phase chromatography, and the fraction containing erinacine A was further purifies in the second dimension by semi-preparative reversed-phase chromatography. This strategy utilises the orthogonality of the two chromatographic modes of effectively eliminate difficult impurities, including structural isomers and analogues of erinacine A.<\/p>\n<p data-start=\"829\" data-end=\"945\">Complementary analytical approaches such as: high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography with ultraviolet and tandem mass spectrometric detection (HPLC-UV-MS\/MS) were employed to unambiguously confirm erinacine A in the isolated fractions, while HPLC with a charged aerosol detector (CAD) was used to determine its purity.<\/p>\n<p>Given the limited commercial availability and the high price of erinacine A, the described method offers a straightforward and cost-effective alternative to obtain this valuable compound for further research and applications.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-11680\" src=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/HPLC_CAD-chromatograms-of-erinacine-A-300x214.jpg\" alt=\"\" width=\"604\" height=\"431\" srcset=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/HPLC_CAD-chromatograms-of-erinacine-A-300x214.jpg 300w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/HPLC_CAD-chromatograms-of-erinacine-A-287x205.jpg 287w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/HPLC_CAD-chromatograms-of-erinacine-A.jpg 560w\" sizes=\"auto, (max-width: 604px) 100vw, 604px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A simple and robust two-dimensional chromatographic fractionation protocol for the isolation of the neuroprotective compound erinacine A from Hericium erinaceus is proposed. This production platform yielded 19.4 mg of erinacine A from approximately 130 g of mushroom material, with a chromatographic purity of 97.4%. The procedure includes extraction, concentration, fractionation, purification, and characterisation of the [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":11694,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1072],"tags":[1484,865,1312,866,1485],"class_list":["post-11686","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-studies","tag-2d-heart-cut-chromatography","tag-erinacin-a","tag-food-supplement","tag-hericium-erinaceus","tag-isolate"],"acf":[],"_links":{"self":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/comments?post=11686"}],"version-history":[{"count":4,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11686\/revisions"}],"predecessor-version":[{"id":11936,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11686\/revisions\/11936"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media\/11694"}],"wp:attachment":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media?parent=11686"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/categories?post=11686"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/tags?post=11686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}