{"id":11560,"date":"2026-05-12T07:43:44","date_gmt":"2026-05-12T06:43:44","guid":{"rendered":"https:\/\/goba.eu\/uporaba-pivovarskih-tropin-za-gojenje-glive-pleurotus-ostreatus-in-proizvodnjo-encimov\/"},"modified":"2026-05-19T12:17:56","modified_gmt":"2026-05-19T11:17:56","slug":"the-use-of-spent-brewery-grains-for-pleurotus-ostreatus-cultivation-and-enzyme-production","status":"publish","type":"post","link":"https:\/\/goba.eu\/en\/the-use-of-spent-brewery-grains-for-pleurotus-ostreatus-cultivation-and-enzyme-production\/","title":{"rendered":"The use of spent brewery grains for Pleurotus ostreatus cultivation and enzyme production"},"content":{"rendered":"<div class=\"qMYqUG_convSearchResultHighlightRoot\">\n<div class=\"relative w-full overflow-visible\">\n<section class=\"text-token-text-primary w-full focus:outline-none [--shadow-height:45px] has-data-writing-block:pointer-events-none has-data-writing-block:-mt-(--shadow-height) has-data-writing-block:pt-(--shadow-height) [&amp;:has([data-writing-block])&gt;*]:pointer-events-auto [content-visibility:auto] supports-[content-visibility:auto]:[contain-intrinsic-size:auto_100lvh] R6Vx5W_threadScrollVars scroll-mb-[calc(var(--scroll-root-safe-area-inset-bottom,0px)+var(--thread-response-height))] scroll-mt-[calc(var(--header-height)+min(200px,max(70px,20svh)))]\" dir=\"auto\" data-turn-id=\"request-WEB:62201120-5fd1-4c73-a11f-876ada2abc1e-3\" data-turn-id-container=\"request-WEB:62201120-5fd1-4c73-a11f-876ada2abc1e-3\" data-testid=\"conversation-turn-8\" data-scroll-anchor=\"false\" data-turn=\"assistant\">\n<div class=\"text-base my-auto mx-auto pb-10 [--thread-content-margin:var(--thread-content-margin-xs,calc(var(--spacing)*4))] @w-sm\/main:[--thread-content-margin:var(--thread-content-margin-sm,calc(var(--spacing)*6))] @w-lg\/main:[--thread-content-margin:var(--thread-content-margin-lg,calc(var(--spacing)*16))] px-(--thread-content-margin)\">\n<div class=\"[--thread-content-max-width:40rem] @w-lg\/main:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group\/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn\">\n<div class=\"flex max-w-full flex-col gap-4 grow\">\n<div class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal outline-none keyboard-focused:focus-ring [.text-message+&amp;]:mt-1\" dir=\"auto\" tabindex=\"0\" data-message-author-role=\"assistant\" data-message-id=\"1e1412f5-8ab4-4d1e-a17d-545a46600906\" data-message-model-slug=\"gpt-5-5\" data-turn-start-message=\"true\">\n<div class=\"flex w-full flex-col gap-1 empty:hidden\">\n<div class=\"markdown prose dark:prose-invert wrap-break-word w-full light markdown-new-styling\">\n<p data-start=\"0\" data-end=\"310\">The best substrate mixture for <em>P. ostreatus<\/em> mycelium growth comprised 30 % wheat bran (WB), 68 % beech sawdust (BS) and 2 % CaCO3. On the substrates containing SBG, the fastest mycelium growth was observed on the substrate composed of 10 % SBG, 20 % WB, 68 % BS and 2 % CaCO3.<\/p>\n<p data-start=\"0\" data-end=\"310\">\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-11552\" src=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.14.36-300x270.jpg\" alt=\"\" width=\"392\" height=\"353\" srcset=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.14.36-300x270.jpg 300w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.14.36-287x258.jpg 287w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.14.36.jpg 431w\" sizes=\"auto, (max-width: 392px) 100vw, 392px\" \/><\/p>\n<p data-start=\"0\" data-end=\"339\">According to these results, substrates with the addition of SPBs could be an appropriate substrate for <em>Pleurotus ostreatus<\/em> cultivation. It can be concluded that the substrate-containing 20 % WB, 10 % SBG and 2 % CaCO3 with 65 % water content, resulting in 51 % biological efficiency, is the most appropriate one for cultivating strain Pl.04.<\/p>\n<p data-start=\"0\" data-end=\"339\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-11549\" src=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.07.13-300x246.jpg\" alt=\"\" width=\"393\" height=\"322\" srcset=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.07.13-300x246.jpg 300w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.07.13-287x235.jpg 287w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.07.13.jpg 430w\" sizes=\"auto, (max-width: 393px) 100vw, 393px\" \/><\/p>\n<div class=\"t m0 x35 hc yfe ffa fs9 fc0 sc0 ls8 ws12c\">The substrate-containing 10 % SBG, 20 % WB, 68 % BS and 2 % CaCO3 overgrown with <em><span class=\"ff2 ws12e\">P. ostreatus <\/span><\/em><span class=\"ws12f\">mycelium could be used for <\/span>other applications, for example as animal feed or as mycoremediation media, because of its excellent mycelium growth promoting properties.<\/div>\n<div><\/div>\n<div>In further experiments different strains should be evaluated to determine the one most appropriate for cultivation on the substrates containing SBG. Adaptation of different fungal strains for the growth on the SBG-containing substrates should be done as well to potentially increase BE and the quality of fruiting bodies.<\/div>\n<div><\/div>\n<div>This work shows that formulations composed of SBG and WB can also be successfully used for enzyme production. Nevertheless, we noted that a higher WB addition tends to yield higher enzyme activities, for different enzyme types at different SBG supplementation rates.<\/div>\n<p data-start=\"312\" data-end=\"681\" data-is-last-node=\"\" data-is-only-node=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-11550\" src=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.08.45-300x150.jpg\" alt=\"\" width=\"606\" height=\"303\" srcset=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.08.45-300x150.jpg 300w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.08.45-768x384.jpg 768w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.08.45-287x144.jpg 287w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.08.45-600x300.jpg 600w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.08.45.jpg 866w\" sizes=\"auto, (max-width: 606px) 100vw, 606px\" \/>.\u00a0 \u00a0 <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-11551\" src=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.10.06-300x150.jpg\" alt=\"\" width=\"602\" height=\"301\" srcset=\"https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.10.06-300x150.jpg 300w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.10.06-768x384.jpg 768w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.10.06-287x144.jpg 287w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.10.06-600x300.jpg 600w, https:\/\/goba.eu\/wp-content\/uploads\/2026\/05\/Screenshot-2026-05-12-at-08.10.06.jpg 866w\" sizes=\"auto, (max-width: 602px) 100vw, 602px\" \/><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"z-0 flex min-h-[46px] justify-start\"><\/div>\n<div class=\"mt-3 w-full empty:hidden\">\n<div class=\"text-center\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"contents\"><\/div>\n<\/div>\n<\/div>\n<div class=\"pointer-events-none -mt-px h-px translate-y-[calc(var(--scroll-root-safe-area-inset-bottom)-14*var(--spacing))]\" aria-hidden=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The best substrate mixture for P. ostreatus mycelium growth comprised 30 % wheat bran (WB), 68 % beech sawdust (BS) and 2 % CaCO3. On the substrates containing SBG, the fastest mycelium growth was observed on the substrate composed of 10 % SBG, 20 % WB, 68 % BS and 2 % CaCO3. \u00a0 According [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":11562,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1072],"tags":[1354,899,1206,1330,1355,1138],"class_list":["post-11560","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-studies","tag-biological-efficiency","tag-mushroom-cultivation","tag-oyster-mushroom","tag-pleurotus","tag-sbg","tag-spent-brewery-grains"],"acf":[],"_links":{"self":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11560","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/comments?post=11560"}],"version-history":[{"count":7,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11560\/revisions"}],"predecessor-version":[{"id":11943,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/posts\/11560\/revisions\/11943"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media\/11562"}],"wp:attachment":[{"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/media?parent=11560"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/categories?post=11560"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/goba.eu\/en\/wp-json\/wp\/v2\/tags?post=11560"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}